Print

The Dampening Effect of Hyperimmune on Carrageenan-Induced Neutrophil Migration

Structural Research Labs, Mobile, AL.

Walter Wilborn, Ph. D., Terry Pierce, Barbara Hyde and S-C. Xiang

Background:
Within 4-5 hr after injection of carrageenan into the pleural cavity, there is an influx of neutrophils. This migration is inhibited by the use of anti-inflammatory medications such as aspirin or indomethacin.

Study Design:
Three female rats per sample of egg are injected into the pleural cavity with 2 ml of 1% carrageenan to induce migration of neutrophils. In parallel, these animals are injected in their peritoneal cavity with the appropriate dose of immune egg powder supernatant, or with physiological saline (control). Four hr post challenge, rats are sacrificed, and 50 µl of exudate containing leukocytic infiltrates are removed and the number of cells contained in an aliquo are recorded.

Results:
Injection of carrageenan into the pleural cavity elicits the migration of approximately 80-95 leukocytes/ 0.05 sq. mm. In separate two experiments, injection of carrageenan in parallel with injections of conventional egg supernatants, elicited migration of 60-80 leukocytes/ 0.05 sq. mm. In comparison, injection of increasing doses of immune egg supernatants resulted 20 -55 neutrophils/0.5 sq. mm. migrating into the site.